Fig. 3From: Significant but partial lipoprotein lipase functional loss caused by a novel occurrence of rare LPL biallelic variantsFunctional characterization of missense variants LPL p.Val111Leu and p.Arg270His. Western blot analyses assessed LPL expression in HEK293T cell lysates or medium following transfection with wild-type (WT) or variant LPL constructs, under conditions with and without heparin treatment. (a) LPL expression in lysates from cells without heparin treatment. (b) LPL expression in the medium after heparin treatment. (c) LPL expression in lysates from cells with heparin treatment. In each panel, the left subpanel shows a representative blot, while the right subpanel provides quantification of LPL expression, with band intensities normalized to β-actin. ‘p.V111L & p.R270H’ denotes co-transfection with both variant constructs. *, P < 0.05; ***, P < 0.001Back to article page