N | Ceramide | *QC samples | Concentration (µM) | Inter-day | Intra-day | Recovery (%) | LOD (µM) | R2 | Linear Range (µM) | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Precision (CV%) | Accuracy (PE%) | SD | Precision (CV%) | Accuracy (PE%) | SD | ||||||||
1 | CerC16:0 | QCH | 0.27 | 3.09 | 87.93 | 4.07 | 1.90 | 87.27 | 2.49 | 64.82 | 0.00016 | 0.997 | 0.0036–0.46 |
QCM | 0.07 | 4.08 | 98.89 | 1.51 | 4.78 | 97.23 | 1.74 | 47.46 | |||||
QCL | 0.02 | 2.01 | 100.42 | 0.19 | 0.98 | 101.14 | 0.09 | 51.48 | |||||
LLOQ | 0.0036 | 4.21 | 111.41 | 0.09 | 2.52 | 114.70 | 0.06 | 68.62 | |||||
2 | CerC18:0 | QCH | 0.16 | 3.97 | 89.79 | 3.21 | 2.35 | 88.38 | 1.87 | 66.37 | 0.00034 | 0.996 | 0.0021-0.27 |
QCM | 0.04 | 5.23 | 100.03 | 1.18 | 6.87 | 100.19 | 1.55 | 44.12 | |||||
QCL | 0.0099 | 2.98 | 99.50 | 0.17 | 4.12 | 99.29 | 0.23 | 39.97 | |||||
LLOQ | 0.0021 | 2.68 | 100.85 | 0.03 | 2.46 | 102.28 | 0.03 | 59.31 | |||||
3 | CerC22:0 | QCH | 0.96 | 2.75 | 88.72 | 14.63 | 2.97 | 89.08 | 15.87 | 66.61 | 0.00045 | 0.998 | 0.012–1.54 |
QCM | 0.23 | 4.05 | 93.89 | 5.70 | 4.58 | 95.30 | 6.55 | 44.12 | |||||
QCL | 0.06 | 3.64 | 98.89 | 1.35 | 3.48 | 96.74 | 1.26 | 35.20 | |||||
LLOQ | 0.012 | 2.32 | 100.73 | 0.18 | 1.60 | 100.43 | 0.13 | 52.62 | |||||
4 | CerC24:0 | QCH | 0.96 | 3.28 | 88.64 | 17.42 | 3.59 | 88.79 | 19.10 | 66.05 | 0.00045 | 0.998 | 0.012–1.54 |
QCM | 0.23 | 4.37 | 94.15 | 6.16 | 5.15 | 95.21 | 7.36 | 43.53 | |||||
QCL | 0.06 | 4.52 | 99.52 | 1.69 | 3.84 | 96.69 | 1.39 | 36.59 | |||||
LLOQ | 0.012 | 3.97 | 106.53 | 0.33 | 2.19 | 103.37 | 0.18 | 54.44 | |||||
5 | CerC24:1 | QCH | 0.96 | 1.15 | 87.23 | 6.04 | 0.86 | 86.78 | 4.48 | 60.37 | 0.00045 | 0.998 | 0.012–1.54 |
QCM | 0.23 | 5.07 | 95.09 | 7.23 | 5.91 | 94.54 | 8.38 | 40.01 | |||||
QCL | 0.06 | 3.11 | 93.52 | 1.09 | 3.74 | 92.57 | 1.30 | 43.51 | |||||
LLOQ | 0.012 | 2.14 | 101.42 | 0.17 | 2.04 | 101.71 | 0.16 | 52.75 |