Fig. 2From: GLUT4 localisation with the plasma membrane is unaffected by an increase in plasma free fatty acid availabilityRepresentative immunofluorescence microscopy image detailing the dystrophin border identification identified using the red dystrophin stain (A). Image B shows the dystrophin stain alone, which was used to identify the cell boarder carried out in MATLAB (C). Image D shows the GLUT4 stain (green) including the spots identified by MATLAB analysis in yellow, and this is replicated in E but with the inclusion of the 5 × 1 μm concentric rings that follow the contours of the PM as defined by dystrophin stain. The magenta staining within image A and F represents fibre type staining of MHC1. Scale bar represents 25 μmBack to article page