Fig. 5

MAPK inhibitor diminished TLR4 upregulation induced by LPS + PA. a. RAW264.7 cells were treated with LPS 1 ng/ml, PA 100 μM, or LPS 1 ng/ml plus PA 100 μM for 24 h, then TLR4 mRNA in cells was quantified using real-time PCR. The data from three independent experiments are represented as mean ± SD. b. RAW264.7 cells were pretreated with 10 μM SB, 2.5 μM PD, or 2.5 μM SP for 30 min, followed by LPS plus PA treatment for 24 h, then TLR4 expression was quantified by real-time PCR. The data from three experiments were presented. ^*** P < 0.01; ⁺ P > 0.05