Fig. 3

RAW264.7 cells were transfected with 200 pmol TLR4 siRNA or negative control siRNA for 12 h, then cells were treated with 1 ng/ml LPS, 100 μM PA, or LPS plus PA for 24 h, finally MCP-1 secretion was quantified using ELISA. TLR4 knockdown by siRNA was confirmed using real-time PCR (a). MCP-1 secretion was showed by the fold of control (b). ^*** P < 0.01; ^* 0.01 < P < 0.05; ⁺ P > 0.05. The data were represented as mean ± SD from three independent experiments