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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Hypolipidemic activity of Dracocephalum kotschyi involves FOXO1 mediated modulation of PPARγ expression in adipocytes

Fig. 2

Western blot analyses of PPARγ in Dracocephalum kotschyi extract (DKE)-treated 3T3-L1 cells. a to observe DKE’s adipogenic effect, cells were differentiated using DMI medium (high glucose DMEM containing 1 μM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, and 1.5 μg/ml insulin). After 2 days, the DMI medium was replaced with high glucose DMEM containing 10 μg/ml insulin for another 6 days. The expression level of PPARγ was determined in cells treated with 4 μl/ml DKE and/or 1 μM rosiglitazone-supplemented DMI medium and compared to that of the control cells. b to investigate DKE’s lipogenic effect, differentiated cells were treated with 4 μl/ml DKE and/or 1 μM rosiglitazone for 48 h. The data represent means ± SD. *Significantly different from negative control (P < 0.05, n = 3)

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