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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Exercise intervention alters HDL subclass distribution and function in obese women

Fig. 2

Changes in reverse cholesterol efflux capacity and anti-inflammatory function in response to the intervention. [3H-Cholesterol] was effluxed from RAW264.7 cells for 4 h prior to scintillation counting. Cholesterol efflux capacity represents the mean radiolabel present in culture media relative to that of an untreated control (a). HUVEC cells were treated with 10 μg/ml participant HDL prior to 20 ng/ml tumour necrosis factor (TNF) treatment for 8 h. Results are calculated relative to a no-HDL treatment control (b). Cell lysates were harvested and stored in RNAprotect reagent prior to RNA extraction, followed by cDNA synthesis and quantitative real time PCR. Results as percentage changes relative to a baseline. Results are means ± SEM. VCAM, Vascular Cell Adhesion Molecule

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