Skip to main content
Fig. 3 | Lipids in Health and Disease

Fig. 3

From: A20 deubiquitinase controls PGC-1α expression in the adipose tissue

Fig. 3

A20 expression in distinct adipose tissue depots of mice. Samples containing 500 μg total protein (a-d) or 25 ng cDNA (e-g) from subcutaneous (SC), visceral (VI) or brown (BAT) adipose tissue depots from lean or obese mice were used in immunoprecipitation (a-d) or quantitative real-time PCR (e-g) experiments. In a-d, samples were immunoprecipitated employing anti-A20 as primary antibody. The immunoprecipitation procedure used antibodies sufficient to immunodeplete the sample. The immunoprecipitates were separated by SDS-PAGE, transferred to nitrocellulose membranes and blotted with anti-A20 antibody. In a, typical blots are depicted; in b-d the quantification of bands is graphically represented. In e-g, the quantification of A20 mRNA obtained by quantitative real-time PCR is represented graphically. In all experiments n = 6, *p < 0.05 vs. lean

Back to article page