Fig. 2

PGC-1α expression in distinct adipose tissue depots of mice. Samples containing 500 μg total protein (a-d) or 25 ng cDNA (E-G) from subcutaneous (SC), visceral (VI) or brown (BAT) adipose tissue depots from lean or obese mice were used in immunoprecipitation (a-d) or quantitative real-time PCR (e-g) experiments. In a-d, samples were immunoprecipitated employing anti-PGC-1α as primary antibody. The immunoprecipitation procedure used antibodies sufficient to immunodeplete the sample. The immunoprecipitates were separated by SDS-PAGE, transferred to nitrocellulose membranes and blotted with anti-PGC-1α antibody. In a, typical blots are depicted; in b-d the quantification of bands is graphically represented. In e-g, the quantification of PGC-1α mRNA obtained by quantitative real-time PCR is represented graphically. In all experiments n = 5–6, *p < 0.05 vs. lean