Fig. 3From: Heat shock protein 70 promotes lipogenesis in HepG2 cellsHSPA1A overexpression leads to increased expression of lipogenic enzymes. Cells were analysed for total triglyceride (TG) and total cholesterol (TC) levels (a, b, c, d). Data represent the mean ± s.d, ** P < 0.01, * P < 0.05. Cells were infected with an Hsp70-overexpressing lentivirus and then treated with or without PA (0.5 mM) for 24 h. The mRNA expression levels of FAS, SCD, ACC and SREBP1C as well as the lipid oxidation-related enzymes ACOX, CPT-1, and PPARα were determined by real-time PCR (e, f, g, h). All experiments were repeated three times. Data represent the mean ± s.dBack to article page