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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Palmitate-induced Slc2a4/GLUT4 downregulation in L6 muscle cells: evidence of inflammatory and endoplasmic reticulum stress involvement

Fig. 3

Palmitate effects on ER stress signaling in L6 cells. L6 myotubes were treated with 0.5% ethanol (vehicle) for 12 h (control sample, C) or 0.75 mM palmitate for 2, 6 or 12 h. Relative cytosolic (a-f, h, j, and k) or nuclear (g and i) protein content. Protein content was normalized by ACTB (a-f, h, j, and k), or by total protein analysis with Coomassie Blue-stained gel (G and I). Values are means ± SEM of 7 to 8 (a), 6 to 7 (b), 8 to 12 (c), 10 to 11 (d), 7 to 8 (e), 4 to 6 (f), 8 to 12 (g), 6 to 9 (h), 9 to 10 (i), 5 to 7 (j), and 8 to 9 (k) samples; *P < 0.05 and **P < 0.01 versus C; #P < 0.05 versus 2 h, §P < 0.05 versus 6 h; one-way ANOVA, Student–Newman–Keuls as post hoc test

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