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Table 3 Established experiments for extraction process and experimental response

From: Optimization of lipids’ ultrasonic extraction and production from Chlorella sp. using response-surface methodology

Runs

Type

Time (min)

Temperature (°C)

Chloroform/Methanol (v/v)

Lipid content (%)

Essay 1

Essay 2

1

Factorial points

6

30

1/1

15.8

14.4

2

30

30

1/1

13.3

14.2

3

6

60

1/1

8.4

9.3

4

30

60

1/1

7.9

9.4

5

6

30

3/1

13.4

10.3

6

30

30

3/1

11.6

12.7

7

6

60

3/1

8.5

7.4

8

30

60

3/1

12.9

12.5

9

Star points

6

45

2/1

13.8

13.6

10

30

45

2/1

19.1

20.3

11

18

30

2/1

10.4

10

12

18

60

2/1

21.4

22.6

13

18

45

1/1

5.9

7.9

14

18

45

3/1

13.6

13.2

15

Center points

18

45

2/1

7.8

7.4