Runs | Type | Time (min) | Temperature (°C) | Chloroform/Methanol (v/v) | Lipid content (%) | |
---|---|---|---|---|---|---|
Essay 1 | Essay 2 | |||||
1 | Factorial points | 6 | 30 | 1/1 | 15.8 | 14.4 |
2 | 30 | 30 | 1/1 | 13.3 | 14.2 | |
3 | 6 | 60 | 1/1 | 8.4 | 9.3 | |
4 | 30 | 60 | 1/1 | 7.9 | 9.4 | |
5 | 6 | 30 | 3/1 | 13.4 | 10.3 | |
6 | 30 | 30 | 3/1 | 11.6 | 12.7 | |
7 | 6 | 60 | 3/1 | 8.5 | 7.4 | |
8 | 30 | 60 | 3/1 | 12.9 | 12.5 | |
9 | Star points | 6 | 45 | 2/1 | 13.8 | 13.6 |
10 | 30 | 45 | 2/1 | 19.1 | 20.3 | |
11 | 18 | 30 | 2/1 | 10.4 | 10 | |
12 | 18 | 60 | 2/1 | 21.4 | 22.6 | |
13 | 18 | 45 | 1/1 | 5.9 | 7.9 | |
14 | 18 | 45 | 3/1 | 13.6 | 13.2 | |
15 | Center points | 18 | 45 | 2/1 | 7.8 | 7.4 |