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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer

Fig. 3

The effects of fatty acid treatments on the expression levels of Niemann-Pick C1-Like 1 (NPC1L1) protein (a) and its mRNA (b) in Caco-2 cells. Caco-2 cells were incubated in increasing concentrations of indicated fatty acids micellar solutions for 24 h, as described in the Methods and Materials section. Micellar solutions with 0.1% (v/v) DMSO were as follows: 2 μCi/mL [1,2-3H (N)]-cholesterol, 100 μmol/L cholesterol, 0 mmol/L (for control) or 0.1 / 0.5 / 1.0 mmol/L fatty acid (PAM, OLA, LNA, ARA, EPA or DHA), 0.5 mmol/L monoolein, 6.6 mmol/L sodium taurocholate, and 0.1 mmol/L soy PtdCho in cultural medium containing 20% delipidized FBS. Expression levels in A were normalized to that of the control β-actin protein (P < 0.05), and expression values in B were normalized to GAPDH mRNA, a housekeeping gene. The expression level of NPC1L1 protein or mRNA in the control group was set to 1. #p < 0.05, ##p < 0.01 were compared to that in the control cells (Caco-2 cells incubated with micellar solution without any fatty acid). Data were presented as mean ± SD of three independent experiments. PAM: palmitic acid; OLA: oleic acid; LNA: linoleic acid; ARA: arachidonic acid; EPA: eicosapentaenoic acid; DHA: docosahexaenoic acid

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