Fig. 10

Distributions of pixel red intensity. These plots represent the distribution of pixels of different red intensity in identified cells within the stated conditions. Each condition contains a different number of cells, and cell size is also variable, therefore, the pixel intensity data will vary per condition. Each histogram has been normalized to represent ‘count-density’, rather than ‘frequency’ or ‘raw count’; the area of each bin represents the percent of observed pixels within that intensity range, rather than height. High-intensity pixels reflect a region with a high lipid concentration allowing dye to accumulate, suggestive of lipid bodies. -ApoB (pr We would interpret high-intensity pixels as reflecting an area with a high lipid concentration allowing dye to accumulate, suggestive of lipid bodies.e-cultured with rabbit anti-apolipoprotein B antibodies), Chol (cholesterol and 1-palmitoyl-lyso-phosphatidylcholine (LPC) in PBS), CHS (cholesteryl hemisuccinate and LPC in PBS), Erg (ergosterol and LPC in PBS), LDL (low-density lipoprotein in PBS), LPC (LPC in PBS), LPS (lipopolysaccharide in PBS), PBS (phosphate-buffered saline vehicle), Ser (medium contained 10% fetal bovine serum)