Comparative analyses of DHA-Phosphatidylcholine and recombination of DHA-Triglyceride with Egg-Phosphatidylcholine or Glycerylphosphorylcholine on DHA repletion in n-3 deficient mice

Wu, Fang; Wang, Dan-dan; Wen, Min; Che, Hong-xia; Xue, Chang-hu; Yanagita, Teruyoshi; Zhang, Tian-tian; Wang, Yu-ming

doi:10.1186/s12944-017-0623-2

Lipids in Health and Disease

Table 3 Brain fatty acid composition

From: Comparative analyses of DHA-Phosphatidylcholine and recombination of DHA-Triglyceride with Egg-Phosphatidylcholine or Glycerylphosphorylcholine on DHA repletion in n-3 deficient mice

Fatty acid (%)	0 day after weaning		4 days after weaning					7 days after weaning					14 days after weaning
Fatty acid (%)	n-3 Adq	n-3 Def	n-3 Adq	n-3 Def	DHA-PC	DHA-TG + PC	DHA-TG + α-GPC	n-3 Adq	n-3 Def	DHA-PC	DHA-TG + PC	DHA-TG + α-GPC	n-3 Adq	n-3 Def	DHA-PC	DHA-TG + PC	DHA-TG + α-GPC
14:0	0.55 ± .0.04	0.61 ± 0.04	0.63 ± 0.03	0.65 ± 0.04	0.69 ± 0.03	0.60 ± 0.04	0.61 ± 0.03	0.49 ± 0.01	0.44 ± 0.06	0.45 ± 0.03	0.52 ± 0.03	0.56 ± 0.03	0.70 ± 0.03	0.76 ± 0.02	0.66 ± 0.03	0.66 ± 0.04	0.74 ± 0.05
16:0	22.26 ± 0.27	22.54 ± 0.16	21.23 ± 0.40	21.52 ± 0.30	21.07 ± 0.25	21.48 ± 0.18	21.86 ± 0.17	21.17 ± 0.28	21.09 ± 0.21	20.89 ± 0.17	21.13 ± 0.31	21.54 ± 0.15	20.28 ± 0.31	20.34 ± 0.27	20.41 ± 0.21	20.55 ± 0.23	20.73 ± 0.31
18:0	20.53 ± 0.22	19.95 ± 0.23	19.51 ± 0.31	19.32 ± 0.29	20.06 ± 0.25	19.42 ± 0.25	19.59 ± 0.19	19.38 ± 0.18	19.51 ± 0.21	19.64 ± 0.27	19.84 ± 0.20	19.88 ± 0.23	19.17 ± 0.25	19.35 ± 0.31	19.37 ± 0.22	19.72 ± 0.23	19.61 ± 0.28
SFAs	47.84 ± 0.46	47.42 ± 0.36	47.63 ± 0.36	47.48 ± 0.47	48.14 ± 0.40	47.57 ± 0.54	47.35 ± 0.36	46.55 ± 0.44	46.34 ± 0.33	46.69 ± 0.25	46.13 ± 0.27	45.84 ± 0.39	45.79 ± 0.36	45.92 ± 0.44	45.65 ± 0.25	46.69 ± 0.27	45.69 ± 0.25
16:1n-7	1.32 ± 0.09	1.01 ± 0.12	1.35 ± 0.08	1.21 ± 0.08	1.37 ± 0.09	1.26 ± 0.07	1.37 ± 0.06	1.27 ± 0.03	1.24 ± 0.05	1.44 ± 0.04	1.29 ± 0.10	1.26 ± 0.08	1.38 ± 0.07	1.27 ± 0.05	1.34 ± 0.02	1.34 ± 0.05	1.27 ± 0.01
18:1n-9	14.34 ± 0.24	13.04 ± 0.33	14.96 ± 0.39	14.76 ± 0.27	15.36 ± 0.20	14.75 ± 0.25	14.89 ± 0.23	15.16 ± 0.19	14.98 ± 0.19	15.57 ± 0.28	15.01 ± 0.18	15.08 ± 0.13	15.32 ± 0.32	15.48 ± 0.17	15.96 ± 0.26	15.97 ± 0.18	15.41 ± 0.31
18:1n-7	2.78 ± 0.18	2.89 ± 0.14	2.68 ± 0.15	2.74 ± 0.13	2.69 ± 0.14	2.61 ± 0.11	2.53 ± 0.09	2.55 ± 0.19	2.49 ± 0.16	2.41 ± 0.14	2.82 ± 0.10	2.44 ± 0.14	2.57 ± 0.17	2.35 ± 0.06	2.49 ± 0.11	2.68 ± 0.06	2.53 ± 0.08
MUFAs	22.20 ± 0.47	20.76 ± 0.48	21.86 ± 0.29	21.55 ± 0.33	22.31 ± 0.23	22.51 ± 0.18	22.59 ± 0.22	22.54 ± 0.33	22.86 ± 0.31	22.43 ± 0.27	22.50 ± 0.20	22.58 ± 0.30	22.76 ± 0.30	23.37 ± 0.39	23.07 ± 0.19	23.78 ± 0.27	23.39 ± 0.31
18:2n-6	0.35 ± 0.02	0.46 ± 0.03^*	0.42 ± 0.03^a	0.54 ± 0.03^b	0.61 ± 0.02^c	0.58 ± 0.01^bc	0.53 ± 0.02^b	0.49 ± 0.03	0.52 ± 0.02	0.56 ± 0.03	0.54 ± 0.02	0.51 ± 0.02	0.57 ± 0.02	0.89 ± 0.03	0.42 ± 0.03	0.46 ± 0.02	0.39 ± 0.02
20:4n-6	10.19 ± 0.20	11.97 ± 0.27^**	10.20 ± 0.16^a	11.72 ± 0.17^b	10.34 ± 0.18^a	10.17 ± 0.16^a	10.14 ± 0.14^a	10.04 ± 0.21^a	11.70 ± 0.25^b	10.01 ± 0.14^a	9.96 ± 0.27^a	9.97 ± 0.11^a	10.35 ± 0.09^c	11.45 ± 0.09^d	9.32 ± 0.10^b	9.17 ± 0.11^b	8.81 ± 0.10^a
22:4n-6	2.13 ± 0.16	3.32 ± 0.25^**	2.22 ± 0.08^a	3.12 ± 0.05^c	2.80 ± 0.11^b	22.92 ± 0.07^bc	3.02 ± 0.06^bc	2.30 ± 0.11^a	3.05 ± 0.12^b	2.53 ± 0.24^a	2.58 ± 0.07^a	2.42 ± 0.09^a	2.02 ± 0.08^b	3.13 ± 0.07^c	1.79 ± 0.06^ab	1.86 ± 0.08^ab	1.73 ± 0.08^a
22:5n-6	1.40 ± 0.15	7.20 ± 0.19^**	1.30 ± 0.07^a	7.01 ± 0.12^d	4.84 ± 0.09^b	5.55 ± 0.11^c	5.30 ± 0.07^c	1.21 ± 0.12^a	6.57 ± 0.19^c	4.19 ± 0.17^b	4.39 ± 0.18^b	4.44 ± 0.14^b	1.19 ± 0.07^a	6.31 ± 0.12^c	2.44 ± 0.11^b	2.38 ± 0.13^b	2.69 ± 0.09^b
N-6	15.37 ± 0.25	23.75 ± 0.27^**	15.04 ± 0.15^a	23.31 ± 0.19^c	19.58 ± 0.24^b	20.01 ± 0.35^b	19.74 ± 0.17^b	14.79 ± 0.33^a	22.89 ± 0.18^d	18.01 ± 0.31^b	18.97 ± 0.32^bc	18.31 ± 0.21^ab	14.63 ± 0.46^a	22.77 ± 0.69^b	14.05 ± 0.39^a	14.31 ± 0.43^a	14.17 ± 0.26^a
22:6n-3	14.30 ± 0.15	7.50 ± 0.16^**	15.01 ± 0.17^c	7.60 ± 0.15^a	9.31 ± 0.19^b	9.50 ± 0.15^b	9.82 ± 0.24^b	15.54 ± 0.25^d	7.79 ± 0.18^a	12.21 ± 0.21^bc	11.90 ± 0.19^b	12.70 ± 0.20^c	16.20 ± 0.15^c	8.10 ± 0.09^a	16.51 ± 0.09^c	14.72 ± 0.12^b	16.12 ± 0.14^c
N-3	14.50 ± 0.23	8.07 ± 0.19^**	15.49 ± 0.19^c	7.75 ± 0.16^a	9.97 ± 0.18^b	9.91 ± 0.19^b	10.32 ± 0.18^b	16.12 ± 0.23^d	7.91 ± 0.22^a	12.87 ± 0.12^bc	12.40 ± 0.23^b	13.27 ± 0.15^c	16.82 ± 0.15^c	8.26 ± 0.12^a	17.23 ± 0.13^c	15.22 ± 0.14^b	16.75 ± 0.19^c
PUFAs	29.96 ± 0.63	31.82 ± 0.65	30.51 ± 0.43	31.06 ± 0.51	29.55 ± 0.73	29.92 ± 0.50	30.06 ± 0.39	30.91 ± 0.56	30.80 ± 0.39	30.88 ± 0.43	31.37 ± 0.55	31.58 ± 0.36	31.45 ± 0.47	31.03 ± 0.77	31.28 ± 0.38	29.53 ± 0.45	30.92 ± 0.36
N-6/ N-3	1.06 ± 0.02	2.94 ± 0.04^**	0.97 ± 0.01^a	3.02 ± 0.07^c	1.96 ± 0.04^b	2.01 ± 0.03^b	1.91 ± 0.04^b	0.92 ± 0.01^a	2.89 ± 0.07^d	1.40 ± 0.01^b	1.53 ± 0.01^c	1.38 ± 0.01^b	0.87 ± 0.03^b	2.76 ± 0.07^a	0.82 ± 0.03^b	0.94 ± 0.03^b	0.85 ± 0.02^b
Total	100.00	100.00	100.00	100.00	100.00	100.00	100.00	100	100	100	100	100	100	100	100	100	100

Only major Fatty acid methyl esters were presented; thus they do not add up to 100%. EPA (20:5n-3) were not detected (<0.01%, trace). Each parameter was presented as the mean ± SEM (n = 6). * p < 0.05, ** p < 0.01, significant difference compared to the n-3 Adq group at 3 weeks of age determined by Student’s t-test. Different letters indicate significant difference at p < 0.05 among all dietary groups after 3 weeks of age
SFA saturated fatty acid, MUFA monounsaturated fatty acid, PUFA polyunsaturated fatty acid, N-6 n-6 polyunsaturated fatty acids, N-3 n-3 polyunsaturated fatty acids

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