Fig. 3
From: Hepatitis B virus inhibits the in vivo and in vitro synthesis and secretion of apolipoprotein C3
HBV inhibited ApoC3 expression through its X gene. a HepG2 cells were transfected with pCMV-S, pCMV-E, pCMV-C, pCMV-X, pCMV-P and pCMV-tag2B, the changes in luciferase activity were determined using a luminometer at 48 h post-transfection, each experiment was repeated three times. b HepG2.2.15 cells were transfected with the HBX siRNA or the negative control siRNA(siRNA-NC) for 24 h. ApoC3 mRNA level was quantitatively determined by RT-qPCR; c The expression levels of ApoC3 protein in HepG2.2.15 cells were determined by Western blot; d The content of ApoC3 in the supernatant of HepG2.2.15 cells was determined by ELISA. *P < 0.005