Fig. 2From: Butyrate attenuates lipolysis in adipocytes co-cultured with macrophages through non-prostaglandin E2–mediated and prostaglandin E2–mediated pathwaysEffect of pertussis toxin (PTX) on the production of PGE2 induced by 24 h of co-culture using the contact method. The concentration of PGE2 in the co-culture medium was determined by ELISA. The concentration of PGE2 in the medium of non–co-cultured 3T3-L1 or RAW 264.7 cells served as the control. Values from five independent experiments are expressed as the mean ± SD. ***p < 0.001 versus co-culture, and ### p < 0.001 versus co-culture with PTX, as determined by ANOVA and the Tukey-Kramer testBack to article page