Table 2 Characteristics of primers used for qPCR
Gene | GeneBank accession no. | Sense primer (5′-3′) | Antisense primer (5′-3′) | Product size, bp | Primer efficiency | Annealing Temp., °C |
|---|---|---|---|---|---|---|
Reference genes | ||||||
ACTB | NM_031144.2 | GACCTCTATGCCAACACAGT | CACCAATCCACACAGAGTAC | 154 | 1.92 | 60 |
ATP5B | NM_134364.1 | GCACCGTCAGAACTATTGCT | GAATTCAGGAGCCTCAGCAT | 203 | 1.90 | 60 |
CANX | NM_172008.2 | CCAGATGCAGATCTGAAGAC | CTGGGTCCTCAATTTCACGT | 175 | 2.31 | 60 |
MDH1 | NM_033235.1 | CAGACAAAGAAGAGGTTGCC | CGTCAGGCAGTTTGTATTGG | 206 | 1.97 | 60 |
RPL13 | NM_031101.1 | CTTAAATTGGCCACGCAGCT | CTTCTCAACGTCTTGCTCTG | 198 | 1.94 | 60 |
TOP1 | NM_022615.1 | GAAGAACGCTATCCAGAAGG | GCTTTGGGACTCAGCTTCAT | 137 | 2.00 | 60 |
YWHAZ | NM_013011.3 | GACGGAAGGTGCTGAGAAA | GCAGCAACCTCAGCCAAGT | 198 | 2.09 | 60 |
Target genes | ||||||
TTPA | NM_013048.2 | GGAGGTGGAAACTCAACGGAA | AGCAGCAATCTTCTTGGCTACA | 109 | 1.93 | 60 |
CYP3A1 | NM_013105.2 | TGGTAATAGACTTGAGAGAG | GGGCAGATATACATAAGGA | 196 | 1.90 | 56 |
LDLR | NM_175762.2 | ACAGTGTCCTCCCAAGTCCAA | GCAAATGTGGATCTCGTCCTC | 222 | 1.87 | 60 |
SCARB1 | NM_031541.1 | GGTGCCCATCATTTACCAAC | CCCTACAGCTTGGCTTCTTG | 162 | 1.94 | 60 |
LPL | NM_012598.2 | GAGATTTCTCTGTATGGCACA | CTGCAGATGAGAAACTTTCTC | 276 | 1.99 | 60 |