Fig. 2

Evaluation of H9C2 cell viability by CKK-8 (a) and trypan blue exclusion (b) assay. Hypoxia and reoxygenation (H+R) cause a marked reduction in the number of viable cells. This effect was antagonized by Sfrp1-transfected cells before hypoxia and reoxygenation (Sfrp1+H+R). The cytoprotective effects of Sfrp1 were significantly reduced by the Wnt signaling pathway activator, Licl. Median values with interquartile range (box) and range (whiskers). Post-hoc analyses of group differences were assessed with Kruskal-Wallis pairwise comparison. Statistical significance was set at the standard level (p <0.05), and the Bonferroni correction was applied in post-hoc analyses (α = 0.05/6 = 0.0083). (Note:* vs. control P <0.001; # vs. H+R P <0.001; vs. Sfrp1+H+R P <0.001)