Fig. 2
From: MiR-106b exhibits an anti-angiogenic function by inhibiting STAT3 expression in endothelial cells
a-l Flow cytometry analysis and tube formation assay of HUVECs after transfection. a-d X axis represents fluorescence intensity, while Y axis represents counts of HUVECs. M1 labeled interval stands for the FITC fluorescence intensity range identified by flow cytometry. No matter transfected with miR-106b mimic or miR-106b inhibitor, most of the HUVECs were on the left side of M1 interval, meaning that most of the cells were not apoptotic so they can’t be labeled by fluorescence. e-g Less tubes were formed in the miR-106b mimic transfection group than the other two groups, furthermore, the discontinuity of tube wall was more significant than the other two groups as well (of 10× magnification). h-j On the contrary, HUVECs of miR-106b inhibitor transfection group formed more tubes with more intactness than the other two groups (of 25× magnification). k, l Differences between different treatment groups are significant, data are presented as means ± SEM. **P <0.05, n = 4