Fig. 2

FGF-2 was involved in 15-HETE-induced cell phenotypic alterations. Cells were treated with 15-HETE (0.5 μM) for 24 h. a Western blot for FGF-2 expression. b Cells were transferred with FGF-2 siRNAs, α-SMA was analyzed by Western blot. c The efficiency of FGF-2 silence and its effects on α-SMA without 15-HETE treatments were validated. Data consists of the means of three independent experiments ± SEM (n = 3). * p < 0.05 vs. controls; +p <0 .05 vs. 15-HETE