Fig. 4

Protein expression of key insulin signaling components in epididymal fat at 16 weeks. Protein levels of insulin receptor substrate-1 (Irs-1), protein kinase B (Akt2) and glucose transporter 4 (Glut4) in the epididymal fat of rats from normal litters (NL) and small litters (SL) were assessed by western blotting. Representative blots are shown in (a). Relative protein levels were quantified by scanning densitometry of the bands (b). Results are expressed as mean ± S.E.M (n = 6, 4 litters from each group). ^** P < 0.01, ^*** P < 0.001