Table 1 Primers used for quantitative real-time polymerase chain reaction (PCR)
Gene | Primer pairs | Sequence( 5′ → 3′) | GenBank no | Product length(bp) |
|---|---|---|---|---|
CYP7A1 | Sense primer | AAAGCGGGAAAGCAAAGACCA | NM_012942 | 157 |
Antisense primer | AGTTCAAAGCAGGAGAGCATCAGG | |||
CPT-1 | Sense primer | CCGCAAACTGGACCGAGAAGAGAT | AF029875 | 185 |
Antisense primer | TTTGCCTGGGATGCGTGTAGTGTT | |||
PPAR-α | Sense primer | TACCTGTGAACACGATCTGA | NM_013196 | 136 |
Antisense primer | GCTAGTCTTTCCTGCGAGTA | |||
PPAR-γ | Sense primer | TGTGGGGATAAAGCATCAGC | NM_001145366 | 175 |
Antisense primer | CAAGGCACTTCTGAAACCGA | |||
SREBP-1c | Sense primer | AGGAGGCCATCTTGTTGCTT | AF286470 | 126 |
Antisense primer | GTTTTGACCCTTAGGGCAGC | |||
ACC | Sense primer | GGAAGACCTGGTCAAGAAGAAAAT | NM_022193 | 142 |
Antisense primer | CACCAGATCCTTATTATTGT | |||
β-actin | Sense primer | GGCACCACACTTTCTACAAT | NM_031144 | 123 |
Antisense primer | AGGTCTCAAACATGATCTGG | |||
Leptin | Sense primer | TGCCTATCCACAAAGTCCAG | NM_013076.2 | 381 |
Antisense primer | TGCTCAGAGCCACCACCT | |||
FAS | Sense primer | CGGCGTGTGATGGGGCTGGTA | NM_017332 | 145 |
Antisense primer | AGGAGTAGTAGGCGGTGGTGTAGA |