- Open Access
Glutathione deficiency down-regulates hepatic lipogenesis in rats
© Brandsch et al; licensee BioMed Central Ltd. 2010
- Received: 8 April 2010
- Accepted: 19 May 2010
- Published: 19 May 2010
Oxidative stress is supposed to increase lipid accumulation by stimulation of hepatic lipogenesis at transcriptional level. This study was performed to investigate the role of glutathione in the regulation of this process. For that purpose, male rats were treated with buthionine sulfoximine (BSO), a specific inhibitor of γ-glutamylcysteine synthetase, for 7 days and compared with untreated control rats.
BSO treatment caused a significant reduction of total glutathione in liver (-70%), which was attributable to diminished levels of reduced glutathione (GSH, -71%). Glutathione-deficient rats had lower triglyceride concentrations in their livers than the control rats (-23%), whereas the circulating triglycerides and the cholesterol concentrations in plasma and liver were not different between the two groups of rats. Livers of glutathione-deficient rats had lower mRNA abundance of sterol regulatory element-binding protein (SREBP)-1c (-47%), Spot (S)14 (-29%) and diacylglycerol acyltransferase 2 (DGAT-2, -27%) and a lower enzyme activity of fatty acid synthase (FAS, -26%) than livers of the control rats. Glutathione-deficient rats had also a lower hepatic activity of the redox-sensitive protein-tyrosine phosphatase (PTP)1B, and a higher concentration of irreversible oxidized PTP1B than control rats. No differences were observed in protein expression of total PTP1B and the mature mRNA encoding active XBP1s, a key regulator of unfolded protein and ER stress response.
This study shows that glutathione deficiency lowers hepatic triglyceride concentrations via influencing lipogenesis. The reduced activity of PTP1B and the higher concentration of irreversible oxidized PTP1B could be, at least in part, responsible for this effect.
- High Density Lipoprotein
- G6PDH Activity
- Hepatic Lipogenesis
- Buthionine Sulfoximine
- XBP1 mRNA
Non-alcoholic fatty liver disease (NAFLD) affects approximately 20-30% of the population in developed countries and is a common finding in patients with metabolic syndrome [1, 2]. Besides enhanced lipolysis and decreased β-oxidation, NAFLD is supposed to be caused also by stimulated lipogenesis . Sterol regulatory element-binding protein (SREBP)-1c is a key transcription factor in controlling the mRNA expression of genes which determine lipogenesis . Since oxidative stress is generally participating in the development and progression of diabetes and its complications [5–7], it was assumed that triglyceride accumulation in the liver might be, at least in part, induced by oxidative stress . Actually, recent findings showed that human hepatoma HepG2 cells which were treated with H2O2 accumulated triglycerides through up-regulation of genes encoding SREBP-1c and other genes involved in fatty acid metabolism , and experiments from our research group revealed a higher mRNA expression of fatty acid synthase (FAS), glucose-6-phosphate dehydrogenase (G6PDH) and stearoyl-CoA desaturase (SCD)-1 in HepG2 cells treated with pro-oxidant CuSO4 compared to untreated cells . Data from both studies indicate oxidative stress as a stimulator of lipid synthesis in liver. However, in contrast to these findings, low levels of glutathione induced by administration of buthionine sulfoximine (BSO), a specific inhibitor of γ-glutamylcysteine synthetase , have been shown to attenuate ethanol-induced steatosis as well as hepatic triglyceride concentrations in untreated rats . Glutathione is the most abundant thiol antioxidant in mammalian cells that is directly involved in defense of reactive oxygen species and that functions as a cofactor of antioxidant enzymes such as the glutathione peroxidase (GPx) . Although pro-oxidants and many pathological conditions such as inflammatory liver diseases, diabetes and hyperglycemia are accompanied by reduced intracellular levels of glutathione [13–15], the effect of inhibited glutathione synthesis as a model for endogenously produced oxidative stress on lipogenesis is not yet well understood.
This study investigated the effect of glutathione depletion on lipid concentrations in plasma and liver, on expression of genes and activities of enzymes involved in lipid synthesis. Glutathione levels were reduced by administration of BSO. Treatment of animals with BSO has the advantage to lower tissue glutathione levels without any overt toxicity  or any effect on the hepatic microsomal and cytosolic enzymes [16, 17]. Lipid synthesis was investigated at the transcriptional level by the analysis of the mRNA expression of SREBP-1c, the key transcription factor involved in the stimulation of lipogenesis in the liver [18, 19], and of related enzymes involved in lipid synthesis and at the activity level by analysis of lipogenic enzymes FAS and G6PDH.
We assume that protein-tyrosine phosphatase (PTP)1B could play a crucial role in the effect of glutathione depletion on lipid metabolism, because PTP1B is a redox-sensitive protein and it has been identified as a potent inductor of SREBP-1 gene expression and as a novel regulator of lipogenesis . In active PTP1B, the catalytic cysteine Cys215 is reduced [21, 22], whereas oxidation of Cys215 in the presence of mild oxidative conditions causes the reversible formation of a cyclic sulfenamide species and leads to the inhibition of enzyme activity [23, 24]. Reducing agents such as glutathione have been shown to reduce the active site sulfenamide and fully restore the PTP1B activity [23, 24]. On the other hand, in addition to sulfenamide bond formation, glutathionylation of the catalytic cysteine in PTP1B might also protect the enzyme against overoxidation . Based on those findings, we addressed the question whether glutathione depletion had modulated lipid metabolism via altered PTP1B activity. We further investigated the activity of the transcription factor X-box binding protein (XPB)1 that has been identified as a key regulator of the mammalian unfolded protein response as well as a stimulator of hepatic lipogenesis  and the inositol-requiring enzyme (IRE)-1 that transforms the XBP1 mRNA in its active form (XBP1s) by splicing.
Body and liver weights, glutathione status and activities of antioxidative enzymes in liver
Body weight, total, reduced and oxidized glutathione and enzyme activities in control and BSO rats.
Final body weight (g)
225 ± 13
204 ± 12*
Liver weight (g)
8.87 ± 0.62
9.17 ± 0.89
Total glutathione (μmol/g)
5.39 ± 0.69
1.61 ± 0.63*
5.28 ± 0.70
1.53 ± 0.61*
0.05 ± 0.02
0.04 ± 0.02
108 ± 46
42 ± 16*
GPx (U/mg protein)
1.69 ± 0.67
1.48 ± 0.45
Glutathione reductase (mU/mg protein)
64.9 ± 10.8
95.2 ± 14.8*
SOD (U/mg protein)
48.6 ± 8.5
56.2 ± 10.4
Catalase (U/mg protein)
1421 ± 222
1307 ± 373
Lipid concentrations in plasma, lipoproteins, and liver
Concentrations of triglycerides and cholesterol in plasma, lipoproteins and liver of control and BSO rats.
1.66 ± 0.47
2.02 ± 0.47
0.79 ± 0.23
0.91 ± 0.38
26.1 ± 2.8
20.2 ± 5.2*
2.24 ± 0.40
2.09 ± 0.42
0.31 ± 0.06
0.34 ± 0.12
1.22 ± 0.17
1.18 ± 0.30
7.82 ± 0.88
6.77 ± 1.40
mRNA concentrations and activities of enzymes involved in hepatic lipogenesis
Activity and protein concentration of PTP1B in liver
This study investigated the effect of glutathione deficiency on lipid metabolism. Cellular GSH may be decreased by administering compounds that react with GSH to form conjugates or that oxidize GSH to GSSG. However, these approaches are limited by lack of specificity of the reagents available. Moreover, the effects obtained are transient, associated with major perturbations of metabolism, or both. In this study we used BSO, a relatively nontoxic inhibitor of γ-glutamylcysteine synthetase, to cause a decrease in glutathione level. The effects observed after treatment with BSO are produced by the reactive species that are formed in normal metabolism. Thus, the BSO model of oxidative stress differs significantly from those in which oxidative stress is produced by radiation or by giving compounds that produce oxidation. As expected, treatment with BSO for 7 days caused a significant depletion of total glutathione in the liver of the rats. The diminished levels of total glutathione were mainly attributable to a reduction of GSH, whereby, in both groups of animals the intracellular level of GSSG was very small. The GSH:GSSG-ratio, which is used as an indicator of the cellular redox state , was markedly reduced in response to BSO treatment. Since glutathione functions as a cofactor of GPx , it appears plausible that the livers of BSO-treated rats showed a compensatory increase of glutathione reductase activity.
Glutathione has multiple functions ranging from antioxidant defense to modulation of immune function and many conditions are related to low glutathione levels [12, 27]. Glutathione concentration has been found to be decreased in chemically induced diabetic animals [e.g. 28,29], and transcriptional level of γ-glutamylcysteine synthetase has been found to be lowered in response to insulin deficiency  and hyperglycemia . BSO-induced glutathione deficiency in this study actually lowered triglyceride concentrations in liver by 23%. These data confirm recent results of Donohue et al.  who found a BSO-induced reduction of triglyceride concentration in livers of healthy rats or rats with ethanol-induced steatosis. The reduced mRNA concentrations of SREBP-1c, S14, DGAT2 and diminished activity of FAS which we observed in the livers of BSO-treated rats indicate that the lower hepatic triglyceride concentration was caused by a diminished lipogenesis. Despite the lower liver lipid concentrations, the concentrations of circulating triglycerides were not lower than those of the control rats. Although there have been published a few studies that used a genetic mouse model of chronic glutathione depletion [e.g. 32-34], none of these studies had characterized the lipid metabolism in those animals. A histological survey revealed no evidence for abnormalities in livers of such knockout mice , albeit hepatic triglycerides were not measured in this context.
In contrast to our results, there are a couple of other studies which observed a stimulation of lipogenesis in response to oxidative stress or ER stress. In particular, experiments with human hepatoma cells (HepG2) showed that oxidative stress induced by H2O2  or copper  leads to triglyceride accumulation in these cells by up-regulation of lipogenic transcription factors and enzymes involved in fatty acid synthesis. In addition, Lin et al.  found that oxidative ER stress which was induced by administration of a high saturated fat diet stimulates lipogenesis in liver of mice, whereas antioxidative cysteine-containing compounds such as n-acetyl cysteine were capable of down-regulating lipogenesis. As previously mentioned, we assume that the BSO model which is a model for endogenously produced oxidative stress in normal metabolism differs significantly from those in which oxidative stress is produced by administered compounds that produce exogenous oxidation. However, it would be an interesting scientific issue, how pro-oxidants would influence lipid metabolism under conditions of glutathione deficiency. Based on our findings, we assume that the reduced activity of PTP1B could by an explanation for the lower triglyceride concentrations in livers of BSO-treated rats. As stated before, PTP1B is a redox-sensitive key enzyme which is capable of stimulating the fatty acid synthesis via activation of SREBP-1 . PTP1B is regulated via peroxide-mediated oxidation of the active-site thiol residue to a sulfonic acid , followed by the reaction with glutathione to a mixed disulfide, termed glutathionylation [37, 38]. Glutathionylation of cysteine in the catalytic centre (Cys 215) of PTP1B leads to a reversible inactivation of the enzyme , but glutathionylation of the catalytic cysteine in PTP1B might also protect the enzyme against irreversible overoxidation . The addition of DTT is normally used to ascertain the amount of reversible oxidized PTP1B . However, our analysis revealed reversible oxidized PTP1B only in livers of untreated rats, whereas treatment with BSO increased the amounts of irreversibly oxidized PTP1B. We also observed considerably lower concentration of glutathionylated PTP1B in the BSO-treated rats, which indicates a lower protection of PTP1B against irreversible overoxidation.
On the other hand, we assume that XBP1 has not contributed to the observed alterations of lipid metabolism in the BSO-treated rats. Despite the observed up-regulation of the kinase IRE1 in livers of rats treated with BSO, the mRNA expression of XBP1 and XBP1s was not altered in these livers. IRE-1 is an ER-localizing proximal sensor of ER stress, and normally induces an unconventional splicing of XBP1 mRNA to generate a mature mRNA encoding the active transcription factor XBP1s [40, 41]. XBP1s has been identified as a key regulator of ER stress response  and a transcriptional activator of lipogenesis . The failing increase of mature XBP1s mRNA expression probably contributed, that we did not observe any triglyceride accumulation in livers of rats treated with BSO compared to the controls.
This study confirmed that short-term reduction of available glutathione under conditions of a normal metabolism lowers the concentration of triglycerides in liver probably by reduction of lipid synthesis. We assume that the reduced hepatic activity of the redox-sensitive PTP1B, the lower concentration of glutathionylated PTP1B and the higher concentration of irreversible oxidized PTP1B in the glutathione-depleted rats could explain, at least in part, the reduced levels of triglycerides in the liver of these animals.
Animals and treatment
Twenty male 5-wk old Sprague-Dawley rats (Charles River, Sulzfeld, Germany) were randomly assigned to 2 groups of 10 each (initial body weight: 169 ± 12 g). All rats were kept individually in Macrolon cages in a room with controlled temperature (22 ± 2°C), relative humidity (50-60%), and light (12:12-h light:dark cycle). The rats were fed 16 g per day of a semi-synthetic diet consisting of (g/kg) casein (200), starch (388), sucrose (200), lard (100), cellulose (50), vitamin and mineral mixture (60), and DL-methionine (2). Vitamins and minerals were supplemented according to recommendations of the American Institute of Nutrition for rat diets . Nipple drinkers allowed free access to water. All experimental procedures described followed established guidelines for the care and use of laboratory animals and were approved by the council of Saxony-Anhalt, Germany (42502/3-468 MLU).
One rat group received L-buthionine-(S,R)-sulfoximine (Sigma-Aldrich, Steinheim, Germany) via drinking water at a concentration of 20 mM yielding an average dose level of 1.77 mmol/kg body mass per day for a total of 7 days. The rats of the control group were offered drinking water without BSO. After 7 days, the rats were killed by decapitation under light anesthesia with diethyl ether. Each rat received 7 g of the diet 4 h before decapitation as short-term food-deprivation significantly down-regulates the transcriptional levels of genes encoding enzymes of the lipid metabolism [44, 45] which were to be measured in this study. Whole blood was collected into heparinized polyethylene tubes and plasma was separated by centrifugation at 1,500 × g for 10 min at 4°C. Livers were excised, washed with ice cold NaCl solution and weighed. Aliquots of liver for RNA isolation were immediately snap frozen in liquid nitrogen and stored at -80°C. Plasma was stored at -20°C. Aliquots of liver were homogenized in ice-cold 0.1 M phosphate buffer (pH 7.4) (1:5, w/v) using a Potter-Elvehjem homogenizer and then centrifuged at 700 × g for 10 min at 4°C. The supernatant was used as crude homogenate. For analysis of enzyme activities in cytosol fractions, aliquots of the crude homogenate were centrifuged at 100,000 × g for 1 h at 4°C.
Analysis of lipids in plasma and liver
Chylomicrons, VLDL, LDL and HDL were separated by step-wise ultracentrifugation (Mikro-Ultracentrifuge, Sorvall Products, Bad Homburg, Germany) by appropriate density cuts (chylomicrons, < 0.95 kg/l; VLDL, 0.95 <p < 1.006 kg/l; LDL, 1.006 <p < 1.040 kg/l; HDL, p > 1.063 kg/l). Plasma densities were adjusted by NaCl and KBr. Chylomicrons were isolated by centrifugation at 100,000 × g for 10 min at 4°C and were removed. The other fractions were separated at 900,000 × g for 1.5 h each at 4°C. Lipids from liver were extracted with a mixture of n-hexane and isopropanol (3:2, v/v) . After evaporation of the solvent, the lipids were dissolved with Triton-X . Concentrations of cholesterol and triglycerides in plasma, lipoproteins and lipid extracts from the liver were determined using enzymatic reagent kits (DiaSys Diagnostic Systems, Holzheim, Germany, Cat.-No. 1.1300 99 90 314 and 1.5760 99 90 314).
Analysis of glutathione in liver
Hepatic concentrations of total glutathione, its reduced and oxidized form were measured by use of a spectrophotometric method . In brief, fresh homogenates were diluted in phosphate buffer, mixed with trichloroacetic acid (10%), and centrifuged at 10,000 × g for 15 min at 4°C. The protein-free supernatant was used for analysis. Calibration was performed using standard curves. For measurement of GSSG, the tissue samples were mixed with 2-vinylpyridin and incubated for 60 min prior to analysis.
Analysis of enzyme activities
Enzyme activities in liver were assayed by spectrophotometric methods. Catalase activity was determined at 25°C using hydrogen peroxide as substrate . One unit of catalase activity is defined as the amount consuming one μmol hydrogen peroxide per min. Total SOD activity was determined according to the method of Marklund and Marklund  with pyrogallol as substrate. One unit of SOD activity is defined as the amount of enzyme required to inhibit the autoxidation of pyrogallol by 50%. GPx activity was determined at 25°C by use of t-butyl hydroperoxide as substrate . One unit of GPx activity is defined as one μmol reduced β-nicotinamide adenine dinucleotide phosphate (NADP) oxidized per min. Glutathione reductase activity was measured according to a method of Carlberg and Mannervik  with one unit defined as the amount of enzyme required for the reduction of one μmol NADPH per min at 25°C. FAS activity was measured in liver cytosol by analyzing the rate of malonyl-CoA-dependent NADPH oxidation . One unit of FAS activity is expressed as one nmol of NADPH oxidized per min at 25°C. G6PDH activity in liver cytosol was assayed by analyzing the rate of NADP reduction under conditions of inhibition of 6-phosphogluconate dehydrogenase by maleimide . One unit of G6PDH activity is defined as one nmol of NADP reduced per min at 25°C. The PTP1B activity in liver homogenate was measured under reducing and non-reducing conditions  with modifications [39, 56] using para-nitrophenyl phosphate (pNPP, extinction coefficient 17.8 l × M-1 × cm-1) as substrate. Native PTP activity was measured under non-reducing conditions. To determine the total PTP activity including the reversible inactive form, the measurement was repeated under reducing conditions by adding 2.5 mmol/l of DTT to the assay buffer. All enzyme activities were normalized to 1 mg of protein. The protein contents of the samples were determined according to the method of Bradford et al. .
Western blot analysis of PTP1B
Total and glutathionylated PTP1B protein abundance in liver tissue was measured by Western blot analysis according to a recently described method  with modifications. For analysis crude homogenates were diluted 1:10 (w/v) in RIPA lysis buffer containing 50 mM Tris-base (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% (v/v) protease inhibitor cocktail (Sigma), 1% (w/v) sodium desoxycholeate, 1% (w/v) Triton X-100, 0.1% (w/v) SDS. The homogenate was centrifuged at 10,000 × g for 30 min at 4° C, and the supernatant was removed and stored at -80° C until further processing. The protein content of the samples was determined by the bicinchoninic acid assay. 40 μg of protein per lane were separated on a 12.5% SDS-polyacrylamide gel  under non-reducing conditions. The separated proteins were transferred to a nitrocellulose membrane (BioTrace™ NT, Pall Corporation, Pensacola, FL, USA) by wet-blotting (90 min at 350 mA). After blocking the membranes in TBST buffer (100 mM Tris-base, pH 7.6, 150 mM NaCl, 0.05% Tween) containing 5% (w/v) non-fat dry milk overnight at 4°C, the blots were incubated with primary antibodies. For total PTP1B protein analysis the mouse anti-PTP1B antibody (BD Biosciences, Pharmingen), and for analysis of the glutathionylated PTP1B protein abundance the mouse anti-GSH antibody (Virogen, Watertown, USA) was used, both diluted 1:2,500 in TBS buffer. Incubation was carried out for 2 h at room temperature. The secondary antibody (goat anti-mouse alkaline phosphatase conjugated antibody, BioFX Lab., Owings Mills, USA) was employed at a dilution of 1:2,500 in TBS buffer for 1 h at room temperature. Antibody binding was visualized with a reaction buffer (100 mM Tris-base, 100 mM NaCl, 50 mM MgCl2) containing 1 M nitro-blue tetrazolium and 1 M 5-bromo-4-chloro-3-indoylphosphate. Optical densities of the bands were evaluated. For normalization a mouse anti-beta-actin antibody (Abcam, Cambridge, UK) was used (1:5,000).
RNA isolation and real-time detection RT-PCR
The RNA was isolated from liver samples using TRIZOL™ reagent (Invitrogen, Karlsruhe, Germany) according to the manufacturer's protocol. Total RNA concentration and purity were estimated from the optical density at 260 and 280 nm, respectively. Synthesis of cDNA and determination of mRNA abundance of SREBP-1c, FAS (EC 220.127.116.11), DGAT-2 (EC 18.104.22.168), S14, PTB1B (EC 22.214.171.124), XBP1 in native and spliced form, IRE-1 and glyceraldehyd-3-phosphat dehydrogenase (GAPDH) were performed by real-time detection PCR as recently described in detail . The primer sequence was for DGAT-2 For-5'-CAGCCCTTAGTGACTCAG-3', Rev-5'-GTGTACAGGAGGCCAGG-3', for FAS For-5'-AGGTGCTAGAGGCCCTGCTA-3', Rev-5'-GTGCACAGACACCTTCCCAT-3', for GAPDH For-5'-GCATGGCCTTCCGTGTTCC-3', Rev-5'-GGGTGGTCCAGGGTTTCTTACTC-3', for IRE-1 For-5'-ACCCACACGGAGACCTTACC-3', Rev-5'-ACTGGTGCCAGCCTTGAGAG-3', for SREBP-1c For-5'-GGAGCCATGGATTGCACATT-3', Rev-5'-AGGAAGGCTTCCAGAGAGGA-3', for S14 For-5'-CCAGCCTCCATCACATCCTTA-3', Rev-5'-CCCCTGGCCGCTTGCTATTAC-3', for XBP1 For-5'-ATTCTGACGCTGTTGCCTCT-3', Rev-5'-CTCTGGGGAAGGACATTTGA-3' and for XBP1s For-5'-GAGTCCGCAGCAGGTG-3', Rev-5'-GTGTCAGAGTCCATGGG-3'. Relative quantification of mRNA abundance of the genes was performed using the ΔΔCt-method with GAPDH as reference gene . Ct-values of the target genes and the reference gene were obtained using RotorGene Software 5.0 (Corbett Research, Mortlake, Australia). Relative mRNA abundance of the genes investigated is expressed as fold change in the BSO group compared to the control group.
Experimental data were analyzed using the Minitab Statistical Software (Minitab, State College, PA, USA). Means of the two groups were compared by Student's t-test, and means of related samples by paired t-test. Means were considered significantly different at P < 0.05.
- Clark JM: The epidemiology of non-alcoholic fatty liver disease in adults. J Clin Gastroenterol. 2006, 40: S5-S10.PubMedGoogle Scholar
- Rector RS, Thyfault JP, Wei Y, Ibdah JA: Non-alcoholic fatty liver disease and the metabolic syndrome: an update. World J Gastroenterol. 2008, 14: 185-192. 10.3748/wjg.14.185PubMed CentralView ArticlePubMedGoogle Scholar
- Postic C, Girard J: The role of the lipogenic pathway in the development of hepatic steatosis. Diabetes Metab. 2008, 34: 643-648. 10.1016/S1262-3636(08)74599-3View ArticlePubMedGoogle Scholar
- Shimomura I, Shimano H, Horton JD, Goldstein JL, Brown MS: Differential expression of exons 1a and 1c in mRNAs for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells. Clin Invest. 1997, 99: 838-845. 10.1172/JCI119247.View ArticleGoogle Scholar
- Ceriello A: Impaired glucose tolerance and cardiovascular disease: the possible role of post-prandial hyperglycemia. Am Heart J. 2004, 147: 803-807. 10.1016/j.ahj.2003.11.020View ArticlePubMedGoogle Scholar
- Baynes JW, Thorpe SR: Role of oxidative stress in diabetic complications: A new perspective on an old paradigm. Diabetes. 1999, 48: 1-9. 10.2337/diabetes.48.1.1View ArticlePubMedGoogle Scholar
- Baynes JW: Role of oxidative stress in development of complications in diabetes. Diabetes. 1991, 40: 405-412. 10.2337/diabetes.40.4.405View ArticlePubMedGoogle Scholar
- Sekiya M, Hiraishi A, Touyama M, Sakamoto K: Oxidative stress induced lipid accumulation via SREBP1c activation in HepG2 cells. Biochem Biophys Res Commun. 2008, 375: 602-607. 10.1016/j.bbrc.2008.08.068View ArticlePubMedGoogle Scholar
- Bettzieche A, Brandsch C, Hirche F, Eder K, Stangl GI: L-cysteine down-regulates SREBP-1c-regulated lipogenic enzymes expression via glutathione in HepG2 cells. Ann Nutr Metab. 2008, 52: 196-203. 10.1159/000138123View ArticlePubMedGoogle Scholar
- Griffith OW: Mechanism of action, metabolism, and toxicity of buthionine sulfoximine and its higher homologs, potent inhibitors of glutathione synthesis. J Biol Chem. 1982, 257: 13704-13712.PubMedGoogle Scholar
- Donohue TM, Curry-MyCoy TV, Todero SL, White RL, Kharbanda KK, Nanji AA, Osna NA: L-buthionine (S, R) sulfoximine depletes hepatic glutathione but protects against ethanol-induced liver injury. Alcohol Clin Exp Res. 2007, 6: 1053-1060. 10.1111/j.1530-0277.2007.00393.x.View ArticleGoogle Scholar
- DeLeve L, Kaplowith N: Glutathione metabolism and its role in hepatotoxicity. Pharmacol Ther. 1991, 52: 287-305. 10.1016/0163-7258(91)90029-LView ArticlePubMedGoogle Scholar
- Griffith OW: Biologic and pharmacologic regulation of mammalian glutathione synthesis. Free Radic Biol Med. 1999, 27: 922-935. 10.1016/S0891-5849(99)00176-8View ArticlePubMedGoogle Scholar
- Lu SC: Regulation of glutathione synthesis. Curr Top Cell Regul. 2000, 36: 95-116. full_textView ArticlePubMedGoogle Scholar
- Franco R, Schoneveld OJ, Pappa A, Panayiotidis MI: The central role of glutathione in the pathophysiology of human diseases. Arch Physiol Biochem. 2007, 113: 234-258. 10.1080/13813450701661198View ArticlePubMedGoogle Scholar
- Watanabe T, Sagisaka H, Arakawa S, Shibaya Y, Watanabe M, Igarashi I, Tanaka K, Totsuka S, Takasaki W, Manabe S: A novel model of continuous depletion of glutathione in mice treated with L-buthionine (S, R)-sulfoximine. J Toxicol Sci. 2003, 28: 455-469. 10.2131/jts.28.455View ArticlePubMedGoogle Scholar
- Drew R, Miners JO: The effects of buthionine sulphoximine (BSO) on glutathione depletion and xenobiotic biotransformation. Biochem Pharmacol. 1984, 33: 2989-2994. 10.1016/0006-2952(84)90598-7View ArticlePubMedGoogle Scholar
- Horton JD, Goldstein JL, Brown MS: SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest. 2002, 109: 1125-1131.PubMed CentralView ArticlePubMedGoogle Scholar
- Shimomura I, Bashmakov Y, Horton JD: Increased levels of nuclear SREBP-1c associated with fatty livers in two mouse models of diabetes mellitus. J Biol Chem. 1999, 274: 30028-30032. 10.1074/jbc.274.42.30028View ArticlePubMedGoogle Scholar
- Shimizu S, Ugi S, Maegawa H, Egawa K, Nishio Y, Yoshizaki T, Shi K, Nagai Y, Morino K, Nemoto K, Nakamura T, Bryer-Ash M, Kashiwagi A: Protein-tyrosine phosphatase 1B as new activator for hepatic lipogenesis via sterol regulatory element-binding protein-1 gene expression. J Biol Chem. 2003, 278: 43095-43101. 10.1074/jbc.M306880200View ArticlePubMedGoogle Scholar
- Barford D, Das AK, Egloff MP: The structure and mechanism of protein phosphatases: insights into catalysis and regulation. Annu Rev Biophys Biomol Struct. 1998, 27: 133-164. 10.1146/annurev.biophys.27.1.133View ArticlePubMedGoogle Scholar
- Barford D, Flint AJ, Tonks NK: Crystal structure of human protein tyrosine phosphatase 1B. Science. 1994, 263: 1397-1404. 10.1126/science.8128219View ArticlePubMedGoogle Scholar
- Salmeen A, Andersen JN, Myers MP, Meng TC, Hinks JA, Tonks NK, Barford D: Redox regulation of protein tyrosine phosphatase 1B involves a sulphenyl-amide intermediate. Nature. 2003, 423: 769-773. 10.1038/nature01680View ArticlePubMedGoogle Scholar
- van Montfort RL, Congreve M, Tisi D, Carr R, Jhoti H: Oxidation state of the active-site cysteine in protein tyrosine phosphatase 1B. Nature. 2003, 423: 773-777. 10.1038/nature01681View ArticlePubMedGoogle Scholar
- Barrett WC, DeGnore JP, König S, Fales HM, Keng YF, Zhang ZY, Yim MB, Chock PB: Regulation of PTP1B via glutathionylation of the active site cysteine 215. Biochemistry. 1999, 38: 6699-6705. 10.1021/bi990240vView ArticlePubMedGoogle Scholar
- Lee AH, Scapa EF, Cohen DE, Glimcher LH: Regulation of hepatic lipogenesis by the transcription factor XBP1. Science. 2008, 320: 1492-1496. 10.1126/science.1158042PubMed CentralView ArticlePubMedGoogle Scholar
- Suthanthiran M, Anderson ME, Sharma VK, Meister A: Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphcytes stimulated via the CD2 and CD3 antigens. Proc Natl Acad Sci USA. 1990, 87: 3343-3347. 10.1073/pnas.87.9.3343PubMed CentralView ArticlePubMedGoogle Scholar
- Rauscher FM, Sanders RA, Watkins JB: Effects of coenzyme Q10 treatment on antoxidant pathways in normal and streptozotocin-induced diabetic rats. J Biochem Mol Tox. 2001, 15: 41-46. 10.1002/1099-0461(2001)15:1<41::AID-JBT5>3.0.CO;2-Z.View ArticleGoogle Scholar
- Melo SS, Arantes MR, Meirelles MS, Jordão AA, Vannucchi H: Lipid peroxidation in nicotinamide-deficient and nictoinamide-supplemented rats with streptozotocin-induced diabetes. Acta Diabetol. 2000, 37: 33-39. 10.1007/s005920070033View ArticlePubMedGoogle Scholar
- Cai J, Sun W, Lu SC: Hormonal and cell density regulation of hepatic γ-glutamylcysteine synthetase heavy and light subunit gene expression. Biochem J. 1995, 326: 167-172.View ArticleGoogle Scholar
- Urata Y, Yamamoto H, Goto S, Tsushima H, Akazawa S, Yamashita S, Nagataki S, Kondo T: Long exposure to high glucose concentration impairs the responsive expression of γ-glutamylcysteine synthetase by interleukin-1β and tumor necrosis factor-α in mouse endothelial cells. J Biol Chem. 1996, 271: 15146-15152. 10.1074/jbc.271.25.15146View ArticlePubMedGoogle Scholar
- Yang Y, Dieter MZ, Chen Y, Shertzer HG, Nebert DW, Dalton TP: Initial characterization of the glutamate-cysteine ligase modifier subunit Gclm(-/-) knockout mouse. Novel model system for a severely compromised oxidative stress response. J Biol Chem. 2002, 277: 49446-49452. 10.1074/jbc.M209372200View ArticlePubMedGoogle Scholar
- Giordano G, Afsharinejad Z, Guizzetti M, Vitalone A, Kavanagh TJ, Costa LG: Organophosphorus insecticides chlorpyrifos and diazinon and oxidative stress in neuronal cells in a genetic model of glutathione deficiency. Toxicol Appl Pharmacol. 2007, 219: 181-189. 10.1016/j.taap.2006.09.016View ArticlePubMedGoogle Scholar
- Kobayashi T, Watanabe Y, Saito Y, Fujioka D, Nakamura T, Obata JE, Kitta Y, Yano T, Kawabata K, Watanabe K, Mishina H, Ito S, Kugiyama K: Mice lacking the glutamate-cysteine ligase modifier subunit are susceptible to myocardial ischaemia-reperfusion injury. Cardiovasc Res. 2010, 85: 785-795. 10.1093/cvr/cvp342View ArticlePubMedGoogle Scholar
- Lin CC, Yin MC, Hsu CC, Lin MP: Effect of five cysteine-containing compounds on three lipogenic enzymes in Balb/cA mice consuming a high saturated fat diet. Lipids. 2004, 39: 843-848. 10.1007/s11745-004-1305-4View ArticlePubMedGoogle Scholar
- Sivaramakrishnan S, Keerthi K, Gates KS: A chemical model for redox regulation of protein tyrosine phosphatase 1B (PTP1B) activity. J Am Chem Soc. 2005, 127: 10830-10831. 10.1021/ja052599eView ArticlePubMedGoogle Scholar
- Denu JM, Dixon JE: Protein tyrosine phosphatases: mechanisms of catalysis and regulation. Curr Opin Chem Biol. 1998, 2: 633-641. 10.1016/S1367-5931(98)80095-1View ArticlePubMedGoogle Scholar
- Denu JM, Tanner KG: Redox regulation of protein tyrosine phosphatases by hydrogen peroxide: detecting sulfenic acid intermediates and examining reversible inactivation. Methods Enzymol. 2002, 348: 297-305. 10.1016/S0076-6879(02)48648-XView ArticlePubMedGoogle Scholar
- Mueller AS, Klomann SD, Wolf NM, Schneider S, Schmidt R, Spielmann J, Stangl G, Eder K, Pallauf J: Redox regulation of protein tyrosine phosphatase 1B by manipulation of dietary selenium affects the triglyceride concentration in rat liver. J Nutr. 2008, 138: 2328-2336. 10.3945/jn.108.089482View ArticlePubMedGoogle Scholar
- Calfon M, Zeng H, Urano F, Till JH, Hubbard SR, Harding HP, Clark SG, Ron D: IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature. 2002, 415: 92-96. 10.1038/415092aView ArticlePubMedGoogle Scholar
- Yoshida H, Matsui T, Yamamoto A, Okada T, Mori K: XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 2001, 107: 881-891. 10.1016/S0092-8674(01)00611-0View ArticlePubMedGoogle Scholar
- Ron D, Walter P: Signal integration in the endoplasmatic reticulum unfolded protein response. Nat Rev Mol Cell Biol. 2007, 8: 519-529. 10.1038/nrm2199View ArticlePubMedGoogle Scholar
- Reeves PG, Nielsen FH, Fahey GC: AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition ad hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. J Nutr. 1993, 123: 1939-1951.PubMedGoogle Scholar
- Shimano H, Yahagi N, Amemiya-Kudo M, Hasty AH, Osuga J, Tamura Y, Shionoiri F, Iizuka Y, Ohashi K, Harada K, Gotoda T, Ishibashi S, Yamada N: Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. J Biol Chem. 1999, 274: 35832-35839. 10.1074/jbc.274.50.35832View ArticlePubMedGoogle Scholar
- Horton JD, Bashmakov Y, Shimomura I, Shimano H: Regulation of sterol regulatory element binding proteins in livers of fasted and refed mice. Proc Natl Acad Sci USA. 1998, 95: 5987-5992. 10.1073/pnas.95.11.5987PubMed CentralView ArticlePubMedGoogle Scholar
- Hara A, Radin NS: Lipid extraction of tissues with a low toxicity solvent. Anal Biochem. 1978, 90: 420-426. 10.1016/0003-2697(78)90046-5View ArticlePubMedGoogle Scholar
- De Hoff JL, Davidson JH, Kritchevsky D: An enzymatic assay for determining free and total cholesterol in tissues. Clin Chem. 1978, 24: 433-435.PubMedGoogle Scholar
- Griffith OW, Meister A: Origin and turnover of mitochondrial glutathione. Proc Natl Acad Sci USA. 1985, 82: 4668-4672. 10.1073/pnas.82.14.4668PubMed CentralView ArticlePubMedGoogle Scholar
- Aebi HE: Catalase. Methods of enzymatic analysis. Edited by: Bergmeyer HU. 1986, 3: 273-286. Weinheim: VCH Verlagsgesellschaft mbH,Google Scholar
- Marklund S, Marklund G: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974, 47: 469-474. 10.1111/j.1432-1033.1974.tb03714.xView ArticlePubMedGoogle Scholar
- Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967, 70: 158-169.PubMedGoogle Scholar
- Carlberg I, Mannervik B: Glutathione reductase. Methods Enzymol. 1985, 113: 484-490. full_textView ArticlePubMedGoogle Scholar
- Nepokroeff CM, Lakshmanan MR, Porter JW: Fatty acid synthase from rat liver. Methods Enzymol. 1975, 35: 37-44. 10.1016/0076-6879(75)35136-7View ArticlePubMedGoogle Scholar
- Deutsch J: Glucose-6-Phosphate dehydrogenase. Methods of enzymatic analysis. Edited by: Bergmeyer HU. 1995, 3: 190-197. Weinheim: VCH Verlag Chemie,Google Scholar
- Zhu L, Goldstein B: Use of an Anaerobic Chamber Environment for the Assay of Endogenous Cellular Protein-Tyrosine Phosphatase Activities. Biol Proced Online. 2002, 4: 1-9. 10.1251/bpo28PubMed CentralView ArticlePubMedGoogle Scholar
- Mueller AS, Bosse AC, Most E, Klomann SD, Schneider S, Pallauf J: Regulation of the insulin antagonistic protein tyrosine phosphatase 1B by dietary Se studied in growing rats. J Nutr Biochem. 2009, 20: 235-247. 10.1016/j.jnutbio.2008.02.007View ArticlePubMedGoogle Scholar
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3View ArticlePubMedGoogle Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. 10.1038/227680a0View ArticlePubMedGoogle Scholar
- Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29: e45- 10.1093/nar/29.9.e45PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.