Table 1 Primer pairs used to amplify PCR products.
From: Postnatal ontogenesis of clock genes in mouse suprachiasmatic nucleus and heart
Gene name | Annealing temperature | GenBank accession no. | Forward primer(5'-3') |
|---|---|---|---|
mBmal1 | 60°C | NM_007489 | Forward:CACTGACTACCAAGAAAGTATG Reverse: ATCCATCTGCTGCCCTGAGA |
mCry1 | 58°C | NM_007771 | Forward:CACTGGTTCCGAAAGGGACTC Reverse: CTGAAGCAAAAATCGCCACCT |
mPer2 | 58°C | NM_011066 | Forward:CAGACTCATGATGACAGAGG Reverse:GAGATGTACAGGATCTTCCC |
Rev-erbα | 61.2°C | NM_145434 | Forward:AGTCGCTGACACTACACAGG Reverse:CCAGGTGGTGAAGGTATCTCC |
GAPDH | 55°C | BC_083149 | Forward:ACAGCCGCATCTTCTTGTGCAGTG Reverse:GGCCTTGACTGTGCCGTTGAATTT |