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Table 2 Experimental protocol: experiments with the hepatocytes.

From: Effects of flavonoids on sphingolipid turnover in the toxin-damaged liver and liver cells

Cells**

Treatments*:

 

Ethanol

Chamiloflan

AP7Glu

CCl4

Un-treated

-

-

-

-

Control (for CCl4-treated)

+

-

-

-

CCl4-treated

-

-

-

+

Control (for flavonoid- treated)

+

-

-

-

Chamiloflan-treated

-

+

-

-

AP7Glu-treated

-

-

+

-

Chamiloflan + CCl4-treated

-

+

-

+

AP7Glu + CCl4-treated

-

-

+

+

Control (for ethanol-treated)

-

-

-

-

Ethanol-treated

+

-

-

-

Chamiloflan + ethanol-treated

+

+

-

-

AP7Glu + ethanol-treated

+

-

+

-

  1. *- The flavonoids and CCl4 were dissolved in the ethanol. The time of incubation with the flavonoids: 4 h and with the CCl4: 2 h. **- Control cell media for the CCl4- and flavonoids-treated hepatocytes contained 30 mM ethanol. Control cell media (for 70 mM ethanol-treated cells) had no additions. Ethanol-treated cell media contained 70 mM ethanol.
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Lipids in Health and Disease

ISSN: 1476-511X