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Table 5 Variation in A) β-actin and B) cyclophilin mRNA levels in five mouse livers submitted to qPCR. Calculations of variations were based on measured Ct value at the same threshold level.

From: Human apoB contributes to increased serum total apo(a) level in LPA transgenic mice

A) β-actin

Within-run (SD)

Between-run (SD)

CV%

No. of observations

Mouse 1

0.02

0.17

0.78

9

Mouse 2

0.11

0.27

1.25

9

Mouse 3

0.09

0.36

1.63

9

Mouse 4

0.09

0.29

1.31

9

Mouse 5

0.23

0.27

1.24

9

All samples

0.22

0.36

1.66

45

B) Cyclophilin

Within-run (SD)

Between-run (SD)

CV%

No. of observations

Mouse 1

0.25

0.18

0.76

6

Mouse 2

0.02

0.06

1.24

6

Mouse 3

0.43

0.32

1.27

6

Mouse 4

0.06

0.26

1.06

6

Mouse 5

0.23

0.17

0.69

6

All samples

0.25

0.27

1.11

30