Figure 5

Immunostaining of CD31 and vWF. The MSCs was induced into endothelial cells which were also transfected with/without SCD1 gene or empty-vector. Immunocytochemical staining was performed using CD31 and vWF antibodies. Positive staining is visualized by red color for vWF (Figure 5a, b, c) or CD31 (Figure 5d, e, f). a/d was referred to SCD1 overexpressed group, b/e was normal group, c/f was empty-vector group. Microscopic image showed that SCD1 overexpressed group (g) had more endothelial cells than normal group (h) and empty-vector group (i).