Skip to main content

Table 6 In vivo and in vitro Cu 2+ -induced generation of malondialdehyde (MDA) in plasma LDL, sd-LDL and lb-LDL from rats after 30 days of methanolic extract (ME) and volatile oil (VO) treatment

From: Elucidation of mechanisms of actions of thymoquinone-enriched methanolic and volatile oil extracts from Nigella sativa against cardiovascular risk parameters in experimental hyperlipidemia

Group

Malondialdehyde†

 

LDL

Sd-LDL

Lb-LDL

 

Basal

Maximal

Basal

Maximal

Basal

Maximal

NLP-C

3.14 ± 0.05

26.69 ± 0.11 (+750.00%)

4.71 ± 0.02

33.02 ± 0.09 (+601.06%)

3.83 ± 0.08

24.73 ± 0.05 (+549.69%)

HLP-C

6.47 ± 0.04 (+106.05%)a

48.40 ± 0.09 (+81.31%)a

8.05 ± 0.06 (+70.91%)a

55.60 ± 0.05 (+66.38%)a

6.18 ± 0.07 (+61.35%)a

32.68 ± 0.06 (+32.14%)a

HLP- ME

4.05 ± 0.08 (-37.40%)b

32.02 ± 0.06 (-33.84%)b

5.78 ± 0.05 (-28.19%)b

39.37 ± 0.06 (-29.19%)b

4.79 ± 0.09 (-22.49%)b

26.73 ± 0.07 (-18.20%)b

HLP-VO

4.80 ± 0.06 (-25.81%)b

36.98 ± 0.03 (-23.59%)b

6.92 ± 0.05 (-14.03%)b

47.08 ± 0.07 (-15.32%)b

5.30 ± 0.04 (-14.23%)b

28.30 ± 0.05 (-13.40%)b

  1. †The values are expressed as nmole/mg protein. The basal MDA values in the table show the in vivo status of oxidized LDL, and its subfractions sd-LDL or lb-LDL. In the case of maximal MDA values of the lipoproteins (LDL and lb-LDL oxidation) were achieved after 2 h of incubation with CuSO4, whereas for sd-LDL maximal values were obtained after 30 min. Each value represents mean ± SD from LDL, sd-LDL and lb-LDL subfractions, isolated from pooled plasma samples in each group. The values were statistically significant from NLP-C at ap < 0.05 and HLP-C at bp < 0.05, and the values between the treated groups were also significant (p < 0.05).