Table 2 Weight % of fatty acids in dried blood spots after BHT treatment and storage by test tube or vacuum sealing

Test tube sealed

37.2 ± 0.5

37.6 ± 1.1

38.1 ± 1.4

39.1 ± 0.9

36.4 ± 1.2

39.8 ± 5.2

37.3 ± 0.7

36.7 ± 0.2

41.2 ± 2.3

24.6 ± 0.3

25.5 ± 0.5

25.2 ± 0.7

24.1 ± 0.2

25.1 ± 0.8

24.7 ± 2.1

25.6 ± 0.8

25.7 ± 0.3

23.9 ± 1.3

29.2 ± 0.2

29.7 ± 0.4

29.0 ± 0.4

28.3 ± 0.7

29.3 ± 1.4

29.3 ± 2.8

30.2 ± 0.6

29.8 ± 0.6

28.2 ± 1.7

5.11 ± 0.12

5.15 ± 0.23

5.36 ± 0.33

4.61 ± 0.33

5.04 ± 0.47

4.86 ± 0.43

4.88 ± 0.32

4.92 ± 0.39

4.60 ± 0.39

6.45 ± 0.04

6.08 ± 0.21

6.12 ± 0.50

6.38 ± 0.46

6.97 ± 0.71

6.87 ± 0.25

6.83 ± 0.54

6.88 ± 0.57

6.51 ± 0.69

0.86 ± 0.06

0.85 ± 0.05

0.80 ± 0.06

0.86 ± 0.05

0.85 ± 0.11

0.85 ± 0.14

0.86 ± 0.04

0.87 ± 0.06

0.81 ± 0.07

1.64 ± 0.04

1.48 ± 0.07

1.55 ± 0.18

1.69 ± 0.11

1.93 ± 0.23

1.78 ± 0.05

1.79 ± 0.22

1.80 ± 0.21

1.66 ± 0.18

34.3 ± 0.1

34.8 ± 0.6

34.3 ± 0.7

32.9 ± 1.0

34.4 ± 1.9

34.2 ± 3.2

35.1 ± 0.6

34.7 ± 1.0

32.8 ± 2.0

11.6 ± 0.2

11.1 ± 0.4

11.1 ± 0.9

11.7 ± 0.8

12.9 ± 1.3

12.5 ± 0.5

12.5 ± 1.0

12.6 ± 1.0

11.9 ± 1.2

Test tube sealed under nitrogen

37.2 ± 0.5

37.3 ± 1.4

35.4 ± 0.3

37.7 ± 1.1

36.0 ± 0.2

36.3 ± 1.1

35.4 ± 0.4

37.1 ± 0.8

36.3 ± 1.4

24.6 ± 0.3^a

25.3 ± 0.5^ab

26.3 ± 0.4^bcd

26.0 ± 0.2^bcd

26.5 ± 0.2^bcd

25.8 ± 1.2^abc

26.7 ± 0.2^bcd

26.9 ± 0.7^cd

27.4 ± 0.5^d

29.2 ± 0.2^ab

29.7 ± 0.6^abc

29.9 ± 0.5^abc

28.7 ± 0.5^a

30.5 ± 0.4^abc

30.6 ± 0.7^bc

31.1 ± 0.4^c

29.7 ± 0.6^abc

29.4 ± 1.2^abc

5.11 ± 0.12^ab

5.29 ± 0.04^a

5.44 ± 0.11^a

4.27 ± 0.09^d

4.61 ± 0.14^cd

5.10 ± 0.30^ab

4.82 ± 0.18^bc

4.37 ± 0.12^d

4.41 ± 0.18^cd

6.45 ± 0.04^abc

6.25 ± 0.08^ab

6.13 ± 0.28^ab

5.96 ± 0.15^a

6.37 ± 0.16^ab

7.04 ± 0.49^c

6.68 ± 0.19^bc

6.07 ± 0.16^ab

6.28 ± 0.21^ab

0.86 ± 0.06^abc

0.81 ± 0.02^abc

0.80 ± 0.06^abc

0.76 ± 0.04^a

0.84 ± 0.05^abc

0.91 ± 0.01^c

0.89 ± 0.04^bc

0.78 ± 0.03^a

0.78 ± 0.05^ab

1.64 ± 0.04^ab

1.60 ± 0.04^a

1.52 ± 0.13^a

1.47 ± 0.05^a

1.56 ± 0.04^a

1.86 ± 0.22^b

1.66 ± 0.08^ab

1.46 ± 0.05^a

1.51 ± 0.05^a

34.3 ± 0.1^abc

35.0 ± 0.6^abc

35.3 ± 0.6^bc

33.0 ± 0.6^a

35.1 ± 0.5^bc

35.7 ± 0.4^bc

35.9 ± 0.6^c

34.1 ± 0.7^abc

33.8 ± 1.4^ab

11.6 ± 0.2^ab

11.2 ± 0.1^ab

11.2 ± 0.4^ab

10.6 ± 0.3^a

11.5 ± 0.3^ab

12.9 ± 1.0^c

12.1 ± 0.4^bc

11.0 ± 0.3^ab

11.3 ± 0.4^ab

Vacuum sealed

37.2 ± 0.5

36.5 ± 0.3

37.6 ± 1.1

37.5 ± 1.7

36.7 ± 0.5

35.3 ± 0.6

35.6 ± 0.5

36.7 ± 0.9

36.9 ± 2.2

24.6 ± 0.3^a

26.3 ± 0.1^b

26.7 ± 0.5^b

26.0 ± 0.4^ab

26.7 ± 1.0^bc

27.8 ± 0.2^c

28.1 ± 0.6^c

27.8 ± 0.6^c

28.0 ± 0.5^c

29.2 ± 0.2

29.6 ± 0.4

28.6 ± 1.2

28.7 ± 0.6

28.2 ± 1.7

30.0 ± 0.1

29.1 ± 0.7

29.1 ± 0.6

28.5 ± 1.7

5.11 ± 0.12^a

5.30 ± 0.16^a

5.17 ± 0.07^a

4.54 ± 0.19^b

4.30 ± 0.20^b

4.59 ± 0.07^b

4.38 ± 0.20^b

4.29 ± 0.06^b

4.18 ± 0.32^b

6.45 ± 0.04^a

6.23 ± 0.16^ab

5.49 ± 0.57^b

6.15 ± 0.19^ab

5.84 ± 0.29^ab

6.28 ± 0.07^ab

6.04 ± 0.20^ab

6.00 ± 0.08^ab

5.87 ± 0.40^ab

0.86 ± 0.06^a

0.78 ± 0.02^ab

0.77 ± 0.05^ab

0.80 ± 0.04^ab

0.76 ± 0.06^ab

0.81 ± 0.02^ab

0.76 ± 0.04^ab

0.73 ± 0.01^b

0.72 ± 0.06^b

1.64 ± 0.04^a

1.58 ± 0.05^abc

1.36 ± 0.14^c

1.60 ± 0.08^ab

1.46 ± 0.08^abc

1.58 ± 0.05^abc

1.51 ± 0.06^abc

1.46 ± 0.01^abc

1.41 ± 0.10^ab

34.3 ± 0.1

34.9 ± 0.5

33.8 ± 1.1

33.3 ± 0.8

32.5 ± 1.9

34.6 ± 0.2

33.5 ± 0.9

33.5 ± 0.6

32.7 ± 2.0

11.6 ± 0.2^a

11.3 ± 0.3^ab

10.1 ± 1.0^b

11.2 ± 0.4^ab

10.6 ± 0.6^ab

11.5 ± 0.2^ab

11.0 ± 0.4^ab

10.8 ± 0.2^ab

10.6 ± 0.7^ab

Nitrogen dried vacuum sealed

37.2 ± 0.5

34.8 ± 0.9

35.9 ± 0.1

37.3 ± 1.2

37.6 ± 1.7

35.7 ± 0.5

36.0 ± 1.5

37.9 ± 3.2

35.4 ± 0.7

24.6 ± 0.3^a

27.2 ± 0.1^bcd

27.0 ± 0.2^bc

26.6 ± 0.7^b

27.0 ± 0.4^bc

28.1 ± 0.3^de

27.7 ± 0.5^cd

28.2 ± 0.3^de

28.7 ± 0.3^e

29.2 ± 0.2

30.1 ± 0.5

29.4 ± 0.1

28.7 ± 0.9

28.4 ± 0.9

29.7 ± 0.2

29.1 ± 0.8

27.7 ± 2.3

29.5 ± 0.3

5.11 ± 0.12^a

5.10 ± 0.17^a

5.10 ± 0.29^a

4.41 ± 0.10^b

4.31 ± 0.14^b

4.46 ± 0.11^ab

4.38 ± 0.21^b

4.01 ± 0.50^b

4.34 ± 0.13^b

6.45 ± 0.04

6.24 ± 0.22

6.03 ± 0.03

6.02 ± 0.26

5.98 ± 0.19

6.16 ± 0.11

6.06 ± 0.29

5.70 ± 0.54

6.12 ± 0.14

0.86 ± 0.06^a

0.80 ± 0.04^ab

0.76 ± 0.02^ab

0.77 ± 0.02^ab

0.73 ± 0.04^b

0.77 ± 0.02^ab

0.75 ± 0.04^ab

0.68 ± 0.08^b

0.75 ± 0.03^ab

1.64 ± 0.04

1.56 ± 0.10

1.49 ± 0.02

1.50 ± 0.09

1.49 ± 0.04

1.52 ± 0.06

1.52 ± 0.07

1.37 ± 0.16

1.47 ± 0.04

34.3 ± 0.1

35.2 ± 0.6

34.5 ± 0.2

33.1 ± 1.0

32.7 ± 1.0

34.2 ± 0.3

33.5 ± 1.0

31.7 ± 2.8

33.8 ± 0.5

11.6 ± 0.2

11.3 ± 0.5

10.9 ± 0.1

11.0 ± 0.4

10.8 ± 0.4

11.2 ± 0.2

11.0 ± 0.5

10.1 ± 1.4

11.1 ± 0.3