CD36 haplotypes are associated with lipid profile in normal-weight subjects

Table 5 - Characteristics of the polymorphisms studied in the CD36 gene

Polymorphisms	Primer sequence	Annealing temp. (°C)	Product size (bp)	Restriction enzyme/allele sizes
-33137A/G	F: 5′-CATGCAGCTCTGTTTTATGTGAG-3′	60	159	MseI
-33137A/G	F: 5′-CATGCAGCTCTGTTTTATGTGAG-3′			AA 67, 56, 29, 7
(rs1984112)	R: 5′-CCCCATCTCTTAGGCCCGTGACA-3′			AG 85, 67, 56, 29, 7
(rs1984112)	R: 5′-CCCCATCTCTTAGGCCCGTGACA-3′			GG 85, 67, 7
- 31118G/A	F: 5′-CAAAATCACAATCTATTCAAGACCA-3′	58	190	HhaI
- 31118G/A	F: 5′-CAAAATCACAATCTATTCAAGACCA-3′			AA 190
(rs1761667)	R: 5′-TTTTGGGAGAAATTCTGAAGAG-3′			GA 190, 138, 52
(rs1761667)	R: 5′-TTTTGGGAGAAATTCTGAAGAG-3′			GG 138, 52
-22674 T/C	F: 5-′TCTTGCTGGGCCCTGCCCA-3′	68	452	Hpy 188I
-22674 T/C	F: 5-′TCTTGCTGGGCCCTGCCCA-3′			TT 452
(rs2151916)	R: 5′-TGTTTGCCCCAAGTGCTGGGTC-3′			TC 452, 430, 22
(rs2151916)	R: 5′-TGTTTGCCCCAAGTGCTGGGTC-3′			CC 430, 22
27645Ins/Del16	F: 5′-GGGACCATTGGTGATGAGAAGG-3′	68	563	Ins/Ins
			563, 547	Ins/Del
			547	Del/Del
(rs3840546)	R: 5′–TTGGAAAATGCACGGCCAGCA-3′		547	Del/Del
30294G/C	F: 5′-ACGCTTGGCATCTTCAGAATGCT-3′	60	465	Mnll
30294G/C	F: 5′-ACGCTTGGCATCTTCAGAATGCT-3′			GG 331, 134
(rs1049673)	R: 5′TGAACCCCTGCTCAAGAAACAGAGT-3′			GC 331, 265, 134, 66
				CC 265, 134, 66

ISSN: 1476-511X