Figure 6
From: Modulating the inflammatory properties of activated microglia with Docosahexaenoic acid and Aspirin
50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with TLR agonists. At 24 hours post TLR stimulation, supernatants were collected; total incubation time was 48 hours. Supernatants were assayed for total Nitric Oxide content using a total Nitric Oxide kit. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); PIC = Poly I:C (1μg/mL); DHA = Docosahexaenoic Acid (50μM). ## = p<0.005; ***,### = p<0.0005.