Table 1 Primers used for quantitative RT-PCR analysis
Gene | Product length | Primer sequences |
|---|---|---|
PPARα | 149 bp | F 5’-GATGTCACACAATGCAATTCG -3’ |
R 5’-GGTAGGCTTCGTGGATTCTCT -3’ | ||
TNF-α | 300 bp | F 5’-GGCAGGTCTACTTTGGAGTCATTGC-3’ |
R 5’-ACATTCGAGGCTCCAGTGAATTCGG-3’ | ||
IL-10 | 142bp | F 5’-TGCTGCCTGCTCTTACTGAC-3’ |
R 5’-AGAAAGTCTTCACCTGGCTGA-3’ | ||
OPN | 121 bp | F 5’-GGTGATAGCTTGGCTTATGGAC-3’ |
R 5’-CCTTAGACTCACCGCTCTTCAT-3’ | ||
TGF-β1 | 272 bp | F 5’-CAACGCCATCTATGAGAAAACC-3’ |
R 5’ -ACTGCCGTACAACTCCAGTGAC-3’ | ||
visfatin | 172 bp | F 5’- GGAAAGACCATGAGAAAGATGC-3’ |
R 5’-CTGATGATTAGTGGTGCCTCTG-3’ | ||
PI3K | 198 bp | F 5’- GCACGGCGATTACACTCTTAC-3’ |
R 5’-TTGGACACTGGGTAGAGCAAC-3’ | ||
MMP-2 | 203 bp | F 5’- TTGTGCTGAAAGATACCCTCAA-3’ |
R 5’-CAGGTCAGGTGTGTAACCAATG-3’ | ||
MMP-9 | 242 bp | F 5’-TGAATCAGCTGGCTTTTGTG-3’ |
R 5’-GTGGATAGCTCGGTGGTGTT-3’ | ||
adiponectin | 202 bp | F 5’-CCAGTATCAGGAAAAGAATGTGG-3’ |
R 5’-TGGTGTATGGGCTATGGGTAGT-3’ | ||
HO-1 | 427 bp | F 5’-AACAAGCAGAACCCAGTCTATG-3’ |
R 5’-TGAGCAGGAAGGCGTCTTA-3’ | ||
GAPDH | 233 bp | F 5’-GGTGAAGGTCGGTGTGAACG-3’ |
|  |  | R 5’-CTCGCTCCTGGAAGATGGTG-3’ |