Table 1 Primers for RT-PCR
Gene | Forward primer (5–3) | Reverse primer (5–3) |
|---|---|---|
Glut4 | TCTCAATGGTTGGGAAGGAAA | GAACCGTCCAAGAATGAGTATCTC |
MCK | CGGCTTCACTCTGGACGATG | TCTTATGCTTGTCTGTGGGTTTGT |
myogenin | CCTGGAAGAAAAGGGACTGG | CGCTCAATGTACTGGATGGC |
MHC1 | CTCAAGCTGCTCAGCAATCTATTT | GGAGCGCAAGTTTGTCATAAGT |
MHC2b | CAATCAGGAACCTTCGGAACAC | GTCCTGGCCTCTGAGAGCAT |
FNDC5 | CAGAAGAAGGATGTGCGGATGC | ACAGGCTCACTGGCTGGGCTCT |
CTRP15 | CCCCTTTATCCCATCTGAGGTTCTGC | GGCTACCCGAGGCTGGTGTAGTGAG |
FGF21 | GGAGATCAGGGAGGATGGAAC | TGGCTGTTGGCAAAGAAACC |
IL6 | GTTGCCTTCTTGGGACTGATG | CTGGCTTTGTCTTTCTTGTTATC |
Atrogin1 | CATCCCTGAGTGGCATCG | GAGTCTGGAGAAGTTCCCGTAT |
MuRF1 | ATCCTGCCCTGCCAACAC | CGGAAACGACCTCCAGACAT |
18S | TTGACGGAAGGGCACCACCAG | GCACCACCACCCACGGAATCG |