Figure 4

ERK1/2 signaling pathway was also involved in the process of adiponectin’s anti-apoptosis in H9c2 cells. Cells first were pretreated with 2.5 μg/mL globular adiponectin for 1 h, in the presence or absence of ERK1/2 inhibitor, U0126 (10 μM), and then exposed to 150 μM palmitate for 12 h. Levels of p-ERK1/2 (A and B), total-ERK1/2 (A and B), cleaved caspase-3 (C) and PARP (D) were measured by immunoblot and quantitative analysis. Results were reported as mean ± S.E.M. * P< 0.05 versus control, # P< 0.05 versus PA.