Figure 1

Effects of exercise training concomitant to high-fat diet feeding on IR, IRS1, and Akt phosphorylation and adipoR1/APPL1 protein levels in the adipose tissue. Analysis of insulin-induced tyrosine phosphorylation of IR (A), IRS1 (B), phosphorylation of Akt^Ser473 (C) and ADIPOR1 (D) and APPL1 (E) protein levels. In lower panels, original membrane stripped and reblotted for IR, IRS1,and Akt total protein levels (A, B and C, respectively) and β-actin (D and E). The results are presented as means ± SEM of n = 6, *p < 0.05, different from C-T and C groups with insulin injection versus C-T and C groups with saline injection, ^#p < 0.05 different from DIO versus control groups, and ^$p < 0.05 different from DIO-T versus DIO groups.