Figure 1

In vitro bactericidal activity of chicken sPLA2-IIA. Samples of gram positive bacterial suspensions were taken after incubation with chPLA2-IIA at various concentrations for 20, 60 and 120 min and thereafter cultured on agar. Bacterial viability was assessed by measuring colony forming ability as described in "Materials and Methods". The results shown in the figure are means of two independent tests. The sPLA2 final concentrations were follows: white circle = 15 μg/ml sPLA2-IIA, white triangle = 30 μg/ml sPLA2-IIA, white square = 45 μg/ml sPLA2-IIA, black square = 50 μg/ml sPLA2-IB.