Purification step | Total activity (U) | Specific activity (U/mg) | Yield (%) | Fold |
---|---|---|---|---|
Supernatant | 34380 | 399 | 100 | 1 |
(NH4)2SO4 Precipitation | 30800 | 1294 | 89.6 | 3.24 |
DEAE-Cellulose | 24650 | 1454 | 71.7 | 3.64 |
Sephacryl S-200 | 23540 | 3789 | 68.5 | 9.49 |
Q-Sepharose FF | 14790 | 4150 | 43 | 10.4 |
Sephadex G-100 | 11000 | 5300 | 32 | 13.28 |